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Swedish study: mRNA vaccine can be reverse transcribed into DNA in liver cells.
On February 25, researchers from the Department of Clinical Sciences of Lund University in Switzerland found that the mRNA COVID-19 vaccine developed by Pfizer and BioNTech can be reverse transcribed and integrated into the human genome.
The study, published in the latest issue of the MDPI Medical Journal Network Molecular Biology [2022, 44(3), 1115-1126], confirmed previous rumors that mRNA vaccines have the potential to alter human genes.
A preclinical study of BNT162b2, a COVID-19 mRNA vaccine developed by Pfizer and BioNTech, showed reversible liver effects in animals that received BNT162b2 injections.
Furthermore, a recent study showed that the RNA of SARS-CoV-2 can be reverse transcribed and integrated into the genome of human cells.
In this study, the researchers investigated the effect of BNT162b2 on the human liver cell line Huh7 in vitro.
Exposing Huh7 cells to BNT162b2 and performing quantitative PCR testing on RNA extracted from cells, high levels of BNT162b2 and long interspersed nuclear element 1 (LINE-1) gene expression changes can be detected in Huh7 cells, which are An endogenous reverse transcriptase.
Immunohistochemistry using an antibody that binds to the LINE-1 open reading frame 1 RNA-binding protein (ORFp1) on BNT162b2-treated Huh7 cells indicated increased nuclear distribution of LINE-1.
PCR on the genomic DNA of Huh7 cells exposed to BNT162b2 amplified DNA sequences specific to BNT162b2.
The findings suggest that rapid uptake of BNT162b2 by the human hepatocyte cell line Huh7 results in changes in LINE-1 expression and distribution.
Studies have also shown that BNT162b2 mRNA can be reverse transcribed into DNA in cells as fast as 6 hours after exposure to BNT162b2.
This conclusion partly confirms previous claims that mRNA viruses and vaccines can alter human genes.
The study did not include studies of the consequences of genetic alterations in liver cells.
(source:internet, reference only)