July 12, 2024

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Scientists grow human metaphase kidneys in pigs for the first time

Scientists grow human metaphase kidneys in pigs for the first time



Scientists grow human metaphase kidneys in pigs for the first time.

On Sep 07,  “Cell-Stem Cell” published a new study, showing that they successfully cultivated human-derived metaphase kidneys in pigs.

This is the first report in the world of humanized functional organ heterogeneity In vivo cultivation case.

Scientists grow human metaphase kidneys in pigs for the first time

According to Lai Liangxue, a researcher at the Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, the serious shortage of donor organs has limited the widespread clinical application of organ transplantation, and the in vivo cultivation of xenogeneic organs based on stem cells may be an ideal way to solve this problem in the future.

“The humanized organs obtained through this approach will not only have more comprehensive cell types and more perfect organ structure and function, but also because the donor cells come from the patient’s own body, it will effectively avoid the existence of xenogeneic or allogeneic organ transplantation. immune rejection and other issues.” Lai Liang said.

Previously, there were many technical obstacles in the realization of heterogeneous in vivo cultivation of humanized organs, which led to the failure of the idea of ​​cultivating human organs from pigs.

In this study, a new human induced pluripotent stem cell with high differentiation potential, strong competition and anti-apoptosis ability was used, combined with an optimized embryo compensation technology system, to realize the xenogeneic in vivo cultivation of humanized mesonephros in a kidney-deficient pig model.

Scientists grow human metaphase kidneys in pigs for the first time

In the study, the aforementioned human-derived cells were injected into pig embryos, which were then transplanted into surrogate pigs.

The pig model used was genetically modified to lack genes needed for kidney development, leaving room for transplanted human cells.

The study strictly complied with relevant ethical regulations and international practices, and the pregnancy of surrogate pigs was terminated within 3 to 4 weeks of pig gestational age.

A total of 2 mesonephric chimeric pig embryos with a gestational age of 25 days and 3 mesonephric chimeric pig embryos with a gestational age of 28 days were obtained. Mesonephros means that the kidneys in the body have developed into mesonephros, the second stage of kidney development.

Immunofluorescent staining for key functional genes in kidney development proved that human donor cells had differentiated into functional cells expressing these genes, indicating that human donor cells in pig fetuses would be able to support human Kidney generation.

The researchers stated that this achievement proved for the first time the feasibility of cultivating humanized functional parenchymal organs in xenogeneic large animals based on stem cell and embryo compensation technology, which is a key step for the use of organ defect large animal models for organ xenogeneic in vivo cultivation The first step is of great significance to solving the severe shortage of donor organs.

Scientists grow human metaphase kidneys in pigs for the first time

(source:internet, reference only)


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